KGF

Keratinocyte Growth Factor (KGF, FGF 7, HBGF 7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross reactivity (mouse, monkey, porcine cells) as the other members of the FGF family.

Produced in the endosperm tissue of barley grain (Hordeum vulgare).

PBS pH 7.2, sterile filtered through 0.2 µm filter

Greater than 95% by SDS-PAGE gel analysis.

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the recon statute solution it is recommended to add a carrier protein (0.1% HSA or BSA).

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Each batch of ISOkine growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of ISOkine recombinant human KGF was determined by its dose dependent effect on proliferation of 4MBr5 rhesus monkey epithelial cells. The ED50 for this effect is typically below 10 ng/ml corresponding to specific activity of > 1 x 10e5 U/mg. Optimal concentration should be determined for specific applications and cell lines.

Endotoxin level is less than 0.005ng per µg of ISOkine product (0.05EU/µg).

Purified ISOkine product carries no pyrogenic or proinflammatory contaminants, as assayed with monocyte activation test using Human 10 plex Cytokine Assay measuring IL 6, TNF alpha and IL 1beta induction.** ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland

Recombinant human MESOkine® KGF contains 163 amino acids and a 6 a.a. histidine tag for a total length of 169 a.a. and has a predicted molecular mass of 19.7 kDa. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 24 and 26 kDa in SDS-PAGE