KGF

Keratinocyte Growth Factor (KGF, FGF 7, HBGF 7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross reactivity (mouse, monkey, porcine cells) as the other members of the FGF family.

Recombinant human KGF contains 164 amino acids and a 16 a.a. histidine-based tag for a total length of 180 a.a. and has a predicted molecular mass of 21.2 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 26 and 28 kDa in SDS-PAGE

Produced in the endosperm tissue of barley grain (Hordeum vulgare).

The bioactivity of ISOkine® recombinant human KGF was assayed by measuring its dose dependent effect on proliferation of 4MBr5 rhesus monkey epithelial cells.

The ED50 for this effect is < 10 ng/ml corresponding to specific activity of > 1 x 10e5 U/mg.

We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay. Each batch of ISOkine® growth factor is tested for bioactivity and verified to have comparable activity to a commercial source.

Greater than 95% by SDS-PAGE gel analysis.

Endotoxin level is <0.005ng per µg of ISOkine® product (<0.05EU/µg).

Purified ISOkine® product carries no pyrogenic or proinflammatory contaminants, as assayed with monocyte activation test (MAT Assay) using Human 10 plex Cytokine Assay measuring IL 6, TNF alpha and IL 1 beta induction.

The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Highly stable as supplied for a maximum of 5 years and a minimum of 12 months from date of receipt (check expiration date specific for each lot# on Certificate of Analysis)

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline to a concentration of no less than 100 µg/ml. For long term storage of the recon statute solution it is recommended to add a carrier protein (0.1% HSA or BSA).

PBS pH 7.2, sterile filtered through 0.2 µm filter