MESOkine® EGF, Recombinant

CATALOG NUMBER: MK010H-M001A

 

DESCRIPTION

Epidermal Growth Factor (EGF) is a potent growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation. It stimulates the proliferation of various epidermal and epithelial cells. EGF has been shown to inhibit gastric secretion, promote colony formation of epidermal cells in culture and to be involved in wound healing. EGF is the prototype of a large family of EGF-like proteins, it signals through the c-erbB receptor. Recombinant human EGF is a globular protein with 3 intramolecular disulfide-bonds essential for biological activity. Approximately 70% homology is found between human EGF and EGF isolated from other species. The relative positions of the cysteine residues is conserved.

 

SOURCE

Recombinant MESOkine® EGF is produced in the endosperm tissue of barley seed (Hordeum vulgare).

 

FORMULATION                                                                                                                                                                

Tris pH 7.4, sterile filtered through 0.2 um filter.

 

PURITY

MESOkine® recombinant EGF is purified from barley seeds resulting in an extract containing purified recombinant EGF protein along with co-purified barley seed proteins. The amount of the recombinant EGF protein is typically in the range of 20-30% of total purified protein content. The exact content of the growth factor is stated on the vials. The co-purified barley seed proteins serve as stabilizing proteins that can prolong the lifetime of the recombinant protein and may enhance the bioactivity of growth factors in standardized bioassays.

 

RECONSTITUTION & STABILTY

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline or a solution of your choice, to a concentration of no less than 100 µg/ml. The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

 

BIOLOGICAL ACTIVITY

Each batch of MESOkine® growth factor is tested for bioactivity and verified to have comparable activity to a corresponding commercial source of highly purified (>95%) growth factor. The bioactivity of MESOkine® recombinant human EGF was determined by its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using EGF is typically < 0.1 ng/ml corresponding to specific activity of > 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

 

ENDOTOXIN LEVEL

Endotoxin levels of each MESOkine® is measured and verified to have endotoxin content less than <0.05 EU/ug.

 

MOLECULAR WEIGHT

MESOkine® EGF contains 53 amino acids and an N-terminal 6 a.a. histidine tag for a total length of 59 a.a. and has a predicted molecular mass of 7 kDa. The recombinant protein migrates with an apparent molecular mass of 9.5 kDa in SDS-PAGE.

Regular price 1 mg / €56
BIOLOGICAL ACTIVITY

Each batch of MESOkine® growth factor is tested for bioactivity and verified to have comparable activity to a corresponding commercial source of highly purified (>95%) growth factor. The bioactivity of MESOkine® recombinant human EGF was determined by its dose dependent effect on proliferation of 3T3 cells. The ED50 for this effect using EGF is typically < 0.1 ng/ml corresponding to specific activity of > 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

 

ENDOTOXIN LEVEL

Endotoxin levels of each MESOkine® is measured and verified to have endotoxin content less than <0.05 EU/ug.

 

MOLECULAR WEIGHT

MESOkine® EGF contains 53 amino acids and an N-terminal 6 a.a. histidine tag for a total length of 59 a.a. and has a predicted molecular mass of 7 kDa. The recombinant protein migrates with an apparent molecular mass of 9.5 kDa in SDS-PAGE.