SCF, human, recombinant
SCF, human, recombinant
Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor
Stem Cell Factor (SCF, also known as c-kit ligand, mast cell growth factor and steel factor) is a hematopoietic growth factor acting at the early stages of hematopoiesis. SCF promotes the survival, proliferation and differentiation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also stimulates the growth and activation of mast cells and eosinophils. SCF has been shown to act synergistically with other growth factors, such as GM-CSF, G-CSF, IL-7 and EPO. Human SCF shows low activity on murine cells, while murine and rat SCF are fully active on human cells.
ISOkine™ recombinant human SCF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture.
Sterile filtered through a 0.2 µm filter. Lyophilized from PBS, pH 7.2 PURITY Greater than 95% by SDS-PAGE gel analysis
Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Each batch of ISOkine™ growth factor is tested for bioactivity and verified to have comparable activity to a commercial source. Bioactivity of ISOkine™ recombinant human SCF is measured by its dose-dependent effect on the proliferation of TF-1 cells. The ED50 for this effect is typically between 2 - 10 ng/ml corresponding to specific activity of 1 – 5 x 10e5 U/mg. We recommend that the optimal concentration for each specific application be determined by an initial dose-response assay.
Endotoxin level is less than 0.005ng per µg of ISOkine™ product (0.05EU/µg) as measured by Bacterial Endotoxin Test in compliance with European Pharmacopoeia (2.6.14-Bacterial endotoxin). Ref. Analysis Report from Test Laboratory of Charles River, Ecully, France.
Purified ISOkine™ product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.** ** Ref. The Blood Bank, University Hospital of Iceland, Reykjavik, Iceland
Recombinant human SCF contains 165 amino acids and a 16 a.a. histidine-based tag for a total length of 181 a.a. and has a predicted molecular mass of 20.7 kDa including the histidine-based tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 22-24 kDa in SDS-PAGE.