MESOkine® IL-6, Recombinant

CATALOG NUMBER: MK040H-M001A

 

 

DESCRIPTION

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/ macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9kDa protein containing 185 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells

 

SOURCE

Recombinant MESOkine® IL-6 is produced in the endosperm tissue of barley seed (Hordeum vulgare).

 

FORMULATION                                                                                                                                                                

Tris pH 7.4, sterile filtered through 0.2 um filter.

 

PURITY

MESOkine® recombinant IL-6 is purified from barley seeds resulting in an extract containing purified recombinant IL-6 protein along with co-purified barley seed proteins. The amount of the recombinant IL-6 protein is typically in the range of 20-30% of total purified protein content. The exact content of the growth factor is stated on the vials. The co-purified barley seed proteins serve as stabilizing proteins that can prolong the lifetime of the recombinant protein and may enhance the bioactivity of growth factors in standardized bioassays.

 

RECONSTITUTION & STABILTY

Note: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile buffered saline or a solution of your choice, to a concentration of no less than 100 µg/ml. The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

 

BIOLOGICAL ACTIVITY

Each batch of MESOkine® growth factor is tested for bioactivity and verified to have comparable activity to a corresponding commercial source of highly purified (>95%) growth factor. The bioactivity of MESOkine® recombinant human IL-6 was determined by its dose dependent effect on proliferation of 7TD-1 cells. The ED50 for this effect using IL-6 is typically < 0.1 ng/ml corresponding to specific activity of > 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

 

ENDOTOXIN LEVEL

Endotoxin levels of each MESOkine® is measured and verified to have endotoxin content <0.05 EU/ug.

 

MOLECULAR WEIGHT

Recombinant human MESOkine® IL-6 contains 185 amino acids and a 6 a.a. histidine tag for a total length of 191 a.a. and has a predicted molecular mass of 21.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25 kDa in SDS-PAGE.

Regular price 1 mg / €479
BIOLOGICAL ACTIVITY

Each batch of MESOkine® growth factor is tested for bioactivity and verified to have comparable activity to a corresponding commercial source of highly purified (>95%) growth factor. The bioactivity of MESOkine® recombinant human IL-6 was determined by its dose dependent effect on proliferation of 7TD-1 cells. The ED50 for this effect using IL-6 is typically < 0.1 ng/ml corresponding to specific activity of > 10 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.

 

ENDOTOXIN LEVEL

Endotoxin levels of each MESOkine® is measured and verified to have endotoxin content <0.05 EU/ug.

 

MOLECULAR WEIGHT

Recombinant human MESOkine® IL-6 contains 185 amino acids and a 6 a.a. histidine tag for a total length of 191 a.a. and has a predicted molecular mass of 21.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25 kDa in SDS-PAGE.